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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising and marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been described. Two added splice variants had been documented but detailed nucleotide sequence information just isn't still accessible.

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Blocking peptides are peptides that bind precisely on the focus on antibody and block antibody binding. These peptide commonly contains the epitope recognized via the antibody.

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This information outlines further more possible optimization of mobile and immunoassay parameters to be sure the absolute best results are...

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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...

Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.

Focus on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose customers are characterized by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a fancy with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates on the G1-S stage boundary and is degraded as cells development by means of S section.

The measurement of protein phosphorylation more info is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...

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